vector with sapi (New England Biolabs)
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New England Biolabs
vector with sapi
Vector With Sapi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 954 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/SapI/pm42820952-178-25-28
Average 98 stars, based on 954 article reviews
Vector With Sapi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 954 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/SapI/pm42820952-178-25-28
Average 98 stars, based on 954 article reviews
vector with sapi - by Bioz Stars,
2026-10
98/100 stars
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Sequencing:Article Title: CRISPR-engineered inducible flocculation in Komagataella phaffii enables enhanced biomass separation for biopharmaceutical production Article Snippet: The NANOBODY ® VHH B coding sequence was synthetized by GeneArt (Thermo Fisher Scientific) as a synthetic DNA construct downstream of and in frame with the alpha mating factor (aMF) signal peptide sequence from S. cerevisiae and was delivered cloned into a pMA-RQ plasmid backbone. .. Both plasmids were digested with Plasmid Preparation:Article Title: CRISPR-engineered inducible flocculation in Komagataella phaffii enables enhanced biomass separation for biopharmaceutical production Article Snippet: The NANOBODY ® VHH B coding sequence was synthetized by GeneArt (Thermo Fisher Scientific) as a synthetic DNA construct downstream of and in frame with the alpha mating factor (aMF) signal peptide sequence from S. cerevisiae and was delivered cloned into a pMA-RQ plasmid backbone. .. Both plasmids were digested with Article Title: Poly(A) variants supporting robust transmission stability in bacteria and high protein expression in animals for mRNA in vitro transcription Article Snippet: .. The annealing product was then ligated into the pUC57 vector digested by EcoRI (NEB, Cat# R0101S) and Purification:Article Title: CRISPR-engineered inducible flocculation in Komagataella phaffii enables enhanced biomass separation for biopharmaceutical production Article Snippet: The NANOBODY ® VHH B coding sequence was synthetized by GeneArt (Thermo Fisher Scientific) as a synthetic DNA construct downstream of and in frame with the alpha mating factor (aMF) signal peptide sequence from S. cerevisiae and was delivered cloned into a pMA-RQ plasmid backbone. .. Both plasmids were digested with Gel Extraction:Article Title: CRISPR-engineered inducible flocculation in Komagataella phaffii enables enhanced biomass separation for biopharmaceutical production Article Snippet: The NANOBODY ® VHH B coding sequence was synthetized by GeneArt (Thermo Fisher Scientific) as a synthetic DNA construct downstream of and in frame with the alpha mating factor (aMF) signal peptide sequence from S. cerevisiae and was delivered cloned into a pMA-RQ plasmid backbone. .. Both plasmids were digested with Ligation:Article Title: CRISPR-engineered inducible flocculation in Komagataella phaffii enables enhanced biomass separation for biopharmaceutical production Article Snippet: The NANOBODY ® VHH B coding sequence was synthetized by GeneArt (Thermo Fisher Scientific) as a synthetic DNA construct downstream of and in frame with the alpha mating factor (aMF) signal peptide sequence from S. cerevisiae and was delivered cloned into a pMA-RQ plasmid backbone. .. Both plasmids were digested with Clone Assay:Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development Article Snippet: .. Forward and reverse oligos were designed and Polymerase Chain Reaction:Article Title: An optogenetics-compatible red fluorescent calcium indicator with negligible blue light photoactivation Article Snippet: Plasmid libraries were constructed using 2-fragment Gibson assembly of one vector backbone and one insert (NEBuilder HiFi DNA Assembly, NEB). .. The pDx vectors were digested with SacI-HF (NEB) and other:Article Title: Coordination of sequential RNase activities in an ancient molecular machine Article Snippet: All cloned constructs were sequenced either via Sanger sequencing or full plasmid sequencing at Microsynth Seqlab GmbH. |